Application

Enolase Activity Assay Kit has been used to measure enolase activity.

General description

Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.

Principle

The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λ_ex = 535/λ_em = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H₂O₂ per minute at pH 7.2 at 25 °C.

Suitability

Suitable for the detection of enolase activity in biological samples.